Next Workshops:
May 13-17, 2024 Biophysical Methods Workshop at the National Institutes of Health, Bethesda
For updated event information and registration, see https://www.nibib.nih.gov/news-events/meetings-events/2024-biophysical-methods-protein-interactions
Course Description
This workshop will review the strategies for using hydrodynamic and thermodynamic analytical methods to characterize biological macromolecules and their interactions in solution. We will discuss theoretical and practical aspects of sedimentation equilibrium and sedimentation velocity analytical ultracentrifugation (AUC), dynamic light scattering (DLS), small-angle X-ray scattering (SAXS), isothermal titration calorimetry (ITC), surface plasmon resonance (SPR), and mass photometry (MP).
This week-long workshop will start on Monday, May 13th, at 8:30am and end on Friday, May 17th, at 5pm. We will provide an introduction to the experimental aspects of AUC, DLS, and ITC, including the operation of the instruments, choice of buffers, and the experimental design appropriate for the system under study. This workshop has a strong focus on AUC, and topics such as the assembly of the AUC cells, the operation of the XLA/I analytical ultracentrifuge, as well as advanced applications of AUC to the study of interacting and non-interacting systems will be discussed in detail. Over the course of the workshop, the theory, data analysis, and experimental strategy of other biophysical methods will also be discussed. These lectures will be accompanied by practical computer exercises with the SEDFIT and SEDPHAT data analysis software.
Participant's presentations of their systems of interest and an informal discussion of their data will be an integral part of the workshop.
Target Audience
This workshop is aimed at researchers with at least basic experience in the applications of AUC, DLS, ITC, SPR/BLI, MP, and/or fluorescence techniques for the characterization of biomacromolecules.
Workshop Organizing Committee
Grzegorz Piszczek, Ph.D.
Biophysics Core, NHLBI, NIH
Huaying Zhao, Ph.D.
Laboratory of Dynamics of Macromolecular Assembly, NIBIB, NIH
Workshop Program Advisory Committee
Chad Brautigam, Ph.D.
Department of Biophysics, UTSW Medical Center, Dallas, Texas
Rodolfo Ghirlando, Ph.D.
Laboratory of Molecular Biology, NIDDK, NIH
Alexander Grishaev, Ph.D.
UMD/NIST Institute for Bioscience & Biotechnology Research
Grzegorz Piszczek, Ph.D.
Biophysics Core, NHLBI, NIH
Peter Schuck, Ph.D.
Laboratory of Dynamics of Macromolecular Assembly, NIBIB, NIH
Huaying Zhao, Ph.D.
Laboratory of Dynamics of Macromolecular Assembly, NIBIB, NIH
Workshop History:
The workshops have been held both at the National Institutes of Health in Bethesda and in many international locations, such as the Institut de Biologie Structurale, Grenoble, France, University College London, École Polytechnique Fédérale de Lausanne, Uppsala Unversity, Medical School Hannover, University of Oxford, Centro Investigaciones Biologicas in Madrid, Spain, Yokohama City University, University Kaiserslautern, CNPEM Campinas, Dallas, IIMCB Warsaw, Tsinghua University, and Charles Unversity. Since 2004, more than 900 scientists from academia and industry of more than 20 countries have participated in the workshops, which has advanced numerous research projects and resulted in hundreds of publications.
