May 4-8, 2020 at the National Institutes of Health, Bethesda
By the Foundation for Advanced Education in the Sciences. For more detailed information see https://faes.org/events/biophysical-methods-boot-camp-2020, or contact Peter Schuck (firstname.lastname@example.org)
Workshops for the hydrodynamic and thermodynamic analysis of biological macromolecules and their interactions with SEDFIT and SEDPHAT are being held 2 – 3 times a year, at NIH as part of the bootcamp series of the Foundation for Advanced Education in Sciences (FAES), or hosted in other academic institutions.
The workshops are typically for one week, and consist of lectures on the biophyiscal theory and applications of several biophysical techniques (analytical ultracentrifugation, isothermal titration calorimetry, surface plasmon resonance biosensing, fluorescence spectroscopy), practical exercises in the lab, practical computational analysis exercises, and individual data analysis sessions.
Attendance is limited, and registration is required.
The list of topics will depend on the focus and length of the workshop, usually including the following:
Theory and Practical Data Analysis
For a full length NIH workshop, typically, the first workshop day will have brief introductory lectures for the different methodologies. The practical aspects will be covered in a mixture of hands-on exercises and demonstrations. This will take place largely in our laboratories, alternating with review and discussion of experimental strategies in the seminar room. The following part of the workshop will be devoted to the practical data analysis and take place in a classroom. The goal is to ensure through hands-on training that the participants become familiar with the details of the use of the software, and confident with each type of data analysis. We will have a lecture series in the morning and early afternoon, to introduce and recapitulate the theoretical background as well as the concepts for their implementation in the software, mixed with practical exercises on examples, where each participant will conduct data analyses on his/her laptop concurrent with the step-by-step demonstration projected on the large screen.
From mid afternoon, the participants are expected to give short presentations on a topic of their interest. This is very informal. This can be, for example, a presentation of the biological background of a system of interacting proteins for which sedimentation analysis may be applied in the future; a presentation of an existing application of analytical ultracentrifugation; an introduction to another biophysical technique for studying macromolecules; the presentation of an open question regarding ultracentrifugation technique; the discussion of preliminary data; or simply the introduction of the speaker and her/his interests. The purpose of this section is to learn about areas of application and to stimulate the discussion. In the past, this section has become a favorite for most participants, since it allows to get to know each other's interests, and as a group to take advantage of unique expertise of each of the participants.